Exposure to stomach acid primes Campylobacter for intestinal infection

Campylobacter is a major cause of foodborne gastroenteritis, with an estimated 500,000 infections annually in the UK. The most common infection route is on undercooked poultry meat, and then crossing the lining of the small intestine. To do this, the bacteria must survive the highly acidic conditions in the stomach, and then find a way of getting into the cells that line the small intestine. New research at IFR, strategically funded by the Biotechnology and Biological Sciences Research Council, from the teams of Dr Arnoud van Vliet and Prof Simon Carding shows that not only does Campylobacter have ways of surviving acid shock, it can also respond and adapt to the acidic environment making it better able to colonise the intestine and enter host cells there.

Thanh Le

In the acidic environment in the stomach, the pH can range from approximately 2 to 7, and food can be retained for up to an hour.  Bacteria like E. coli, which are resident in our digestive system, have developed specific acid-resistance systems which Campylobacter doesn’t appear to have, so how it copes with these stressful conditions on entering the stomach is not well understood. To understand more of how Campylobacter reacts to the sudden change in acidity, post graduate student Thanh Le and colleagues have identified which genes are turned on or off by acid shock.

They found that Campylobacter down regulates the activity of genes relating to metabolism and cell division, but up regulates the activity of a set of genes needed to make flagella, the hair-like structures that bacteria use for movement. These changes in gene expression suggest that in addition to slowing down normal cellular processes, acid shock also triggers Campylobacter to become more motile.

As motility and host cell invasion are known to be linked, Le studied how well acid-shocked Campylobacter invaded epithelium obtained from the mouse gut. In this cell model system, the acid shocked Campylobacter were better than unprepared Campylobacter at invading and subsequently getting across this cell layer, two processes essential for disease.

More work is needed to fully understand the mechanism of cell invasion, and how Campylobacter initially senses high acidity is still unknown. But the link between acid shock and increased invasion in Campylobacter highlights how well this dangerous pathogen has adapted to the human host, and such knowledge may help in achieving the ultimate goal of reducing its impact in the food industry.

Reference:

Le MT, Porcelli I, Weight CM, Gaskin DJH, Carding SR, van Vliet AHM (2012) Acid-shock of Campylobacter jejuni induces flagellar gene expression and host cell invasion. European Journal of Microbiology and Immunology 2(1), 12–19. (doi: 10.1556/EuJMI.2.2012.1.3)


 

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